Journal: Reproduction & Fertility
Article Title: Canine endometrial organoids respond to exogenous steroid hormones and are an in vitro model for cystic endometrial hyperplasia
doi: 10.1530/RAF-24-0134
Figure Lengend Snippet: Brightfield images and histological micrographs of healthy canine endometrial organoids. (A) Representative brightfield images of a well of organoids from one individual at passage 3, day 5 (P3D5) following hormone treatments. (B) Representative immunohistochemical micrographs of intact tissue during diestrus and organoids without hormone treatment for vimentin and cytokeratin; negative control is inset micrograph. C, control; E 2 , estrogen only; P 4 , progesterone only; E 2 /P 4 , estrogen for 2 days, then progesterone for 4 days; scale bars = 1 mm (A) and 20 μm (B).
Article Snippet: Next, the slides were incubated with the primary antibody ( ) and biotinylated secondary antibodies (ESR1, FOXO1: Biotin Rb IgG (Vector #BA1000, 1:500 dilution); PGR, Cytokeratin, Vimentin: Biotin Ms IgG (Vector #BA2000, 1:500 dilution)).
Techniques: Immunohistochemical staining, Negative Control, Control